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Proofreading Function of DNA Polymerase

View the animation below, then complete the quiz to test your knowledge of the concept.






1The proofreading function of DNA polymerase reduces the error rate from about one in a million basepairs to about one in a ________ basepairs.
A)hundred thousand
B)ten thousand
C)thousand
D)ten million
E)hundred million



2DNA polymerases use their ________ activity to remove a mismatched basepair.
A)3’ -> 5’ exonuclease
B)5’ -> 3’ exonuclease
C)RNase
D)protease
E)mismatchase



3Proofreading by DNA polymerase involves the removal of
A)only the mismatched base on the old strand of DNA.
B)only the mismatched base on the newly-synthesized strand of DNA.
C)the mismatched basepair on both strands of DNA.
D)several bases on the newly-synthesized strand of DNA.
E)several bases on the old strand of DNA.



4Improper base-pairing during DNA replication causes a pause in chain elongation.
A)True
B)False



5Following base removal, DNA polymerase can add nucleotides in the 5’ to 3’ direction.
A)True
B)False







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