Site MapHelpFeedbackPolymerase Chain Reaction (Quiz 2)
Polymerase Chain Reaction (Quiz 2)

Why is the Polymerase Chain Reaction an Alternative to Radioactive Labeling? The Polymerase Chain Reaction or PCR is one of the most powerful tools of molecular genetics. It uses variation in temperature and a version of DNA polymerase that is stable across a wide temperature range to synthesize many copies of the same DNA sequence in a short period. Radioactive labeling uses a labeled primer to identify the DNA of interest. In PCR the target piece of DNA is amplified (copied over and over) so that is much more common than any unamplified DNA in the sample. Using either technique it is possible to distinguish DNA of interest for other DNA in a sample.

View the animation below, then complete the quiz to test your knowledge of the concept.






1Which of the following hybridize with the ends of the gene to be amplified?
A)Taq polymerase
B)deoxyribonucleotides
C)ribonucleotides
D)DNA molecules
E)DNA primers



2After heating and cooling the mixture, which of the following synthesizes the complementary strands of DNA?
A)Taq polymerase
B)deoxyribonucleotides
C)ribonucleotides
D)DNA molecules
E)DNA primers



3This new complementary strand of DNA is constructed of which of the following?
A)Taq polymerase
B)deoxyribonucleotides
C)ribonucleotides
D)DNA molecules
E)DNA primers



4The polymerase chain reaction is a method for making copies of small amounts of several segments of DNA.
A)True
B)False



5By running the polymerase chain reaction overnight for twenty-one cycles, a single segment of DNA can be amplified to around a thousand copies.
A)True
B)False







Animation Quiz SiteOnline Learning Center

Home > Biology 1 > Chapter 16 > Polymerase Chain Reaction (Quiz 2)