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Polymerase Chain Reaction (Quiz 3)

Why is the Polymerase Chain Reaction an Alternative to Radioactive Labeling? The Polymerase Chain Reaction or PCR is one of the most powerful tools of molecular genetics. It uses variation in temperature and a version of DNA polymerase that is stable across a wide temperature range to synthesize many copies of the same DNA sequence in a short period. Radioactive labeling uses a labeled primer to identify the DNA of interest. In PCR the target piece of DNA is amplified (copied over and over) so that is much more common than any unamplified DNA in the sample. Using either technique it is possible to distinguish DNA of interest for other DNA in a sample.

View the animation below, then complete the quiz to test your knowledge of the concept.






1The purpose of PCR is to
A)make more copies of DNA primers to increase protein synthesis
B)make many copies of an organism’s DNA sequence so a small number of organisms will become large enough to be identified
C)make more RNA so large units of protein can be synthesized
D)recycle DNA using thermocyclers



2For DNA amplification to occur, which of the following are needed?
A)loose ribonucleotides
B)RNA primers
C)thermostable DNA polymerase
D)b and c
E)all of the above



3Taq polymerase starts copying at
A)the end of free single-stranded RNA
B)any open point
C)RNA primers attached to the end of the desired gene
D)DNA primers attached to the end of the desired gene



4Using PCR, over 1 million copies of DNA can be made from a single strand of DNA overnight.
A)True
B)False



5When DNA is heated, primers anneal to DNA strands.
A)True
B)False







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