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Conjugation: The Transfer of Chromosomal DNA

What are the genetic consequences of different types of conjugation? Conjugation can occur whenever the donor bacterial cell has the F+ genotype. The gene can either exist in a plasmid or incorporated into the bacterial chromosome. In either case the donor cell creates a pilus and copies DNA to transfer to the F- cell. If the F+ gene is on a plasmid, then the entire plasmid is transferred and the cell receiving the plasmid becomes F+. However, if the plasmid is incorporated into the chromosome then the process of conjugation attempts to transfer the entire bacterial genome. Only part of the genome is transferred, including a portion of the plasmid and some other genes. The bacterium receiving the genes does not become F+.

View the animation below, then complete the quiz to test your knowledge of the concept.

1F+ refers to a cell containing...
A)a single strand of DNA.
B)a double strand of DNA.
C)the F plasmid.
D)any plasmid.
E)a functional strand of DNA.

2The donor chromosome is transferred as...
A)a plasmid.
B)single stranded DNA.
C)double stranded DNA.
D)single stranded RNA.
E)double stranded RNA.

3HFr refers to a cell that has...
A)integrated the F plasmid into its cytoplasm.
B)integrated the F plasmid into its cell wall.
C)integrated the F plasmid into its sex pilus.
D)integrated the F plasmid into its genome.
E)refused to integrate the F plasmid into the cell.

4The sex pilus is found on the F- cell.

5The entire genome of the donor cell is usually transferred to the recipient cell.

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