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Conjugation: The Transfer of Chromosomal DNA (Quiz 2)

What are the genetic consequences of different types of conjugation? Conjugation can occur whenever the donor bacterial cell has the F+ genotype. The gene can either exist in a plasmid or incorporated into the bacterial chromosome. In either case the donor cell creates a pilus and copies DNA to transfer to the F- cell. If the F+ gene is on a plasmid, then the entire plasmid is transferred and the cell receiving the plasmid becomes F+. However, if the plasmid is incorporated into the chromosome then the process of conjugation attempts to transfer the entire bacterial genome. Only part of the genome is transferred, including a portion of the plasmid and some other genes. The bacterium receiving the genes does not become F+.

View the animation below, then complete the quiz to test your knowledge of the concept.

1HFr refers to
A)a cell with an F plasmid
B)a cell that has combined with the other cells by sexual reproduction
C)a cell in which the F plasmid has been integrated into the cell chromosome
D)a cell that has given away its F plasmid

2In conjugation of an HFr cell with an F- cell
A)the recipient stays F-
B)the recipient becomes F+
C)the recipient becomes HFr
D)the donor becomes F-

3In conjugation, the donor chromosome is transferred as
A)double-stranded DNA
B)single-stranded DNA
C)single-stranded RNA
D)a whole F plasmid
E)double-stranded RNA

4The donor cell DNA is integrated into the recipient cell’s DNA by homologous recombination.

5In conjugation of an HFr cell and an F- cell, the entire genome of the HFr cell is usually transferred to the recipient cell.

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