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PCR Reactions

View the animation below, then complete the quiz to test your knowledge of the concept.






1PCR requires all of the following EXCEPT
A)primers.
B)DNA ligase.
C)DNA polymerase.
D)DNA of interest.
E)deoxyribonucleotides.



2During PCR, which primer anneals to DNA with its 3’ end towards the center of the sequence to be amplified?
A)forward primer only
B)reverse primer only
C)both the forward and reverse primers
D)either the forward or reverse primer, but not both
E)neither the forward nor reverse primer



3Arrange the following in the proper sequence in which they occur during a single PCR cycle.

1. Addition of DNA nucleotides by Taq polymerase
2. Complementary base pairing between primers and target DNA
3. Heat separation of strands of target DNA
A)1, 2, 3
B)2, 1, 3
C)2, 3, 1
D)3, 2, 1
E)3, 1, 2



4All double-stranded DNA molecules generated by PCR are identical.
A)True
B)False



5PCR is carried out in machines termed thermocyclers.
A)True
B)False







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