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DNA Replication (Quiz 3)

Why is a pre-existing DNA strand necessary for the action of DNA polymerase? DNA polymerase uses the complementary nature of DNA strands to replicate DNA. The polymerase enzyme does not contain any information about the order of base sequences. Without a template strand of DNA the enzyme is incapable of selecting which base to add to the sequence next. DNA polymerase binds to a DNA strands and adds new material to it in order to build a complementary strand. The complementary nature of the four bases provides the information to build the correct sequence guanines, cytosines, adenines, and thymines.

View the animation below, then complete the quiz to test your knowledge of the concept.

1Arrange the following proteins in the proper order in which they participate in DNA replication.
1 = Primase
2 = Helicase
3 = Single-strand binding proteins
4 = DNA polymerase
A)1, 2, 3, 4
B)1, 3, 2, 4
C)2, 3, 1, 4
D)2, 3, 4, 1
E)2, 4, 3, 1

2What is the function of the enzyme primase during DNA replication?
A)to unwind the double helix to prime it for replication
B)to prime DNA for replication by preventing the formation of knots
C)to add a DNA primer to enable replication of DNA
D)to add a RNA primer to enable replication of DNA
E)to correct errors made during DNA replication

3The short DNA segments formed on the discontinuously replicated strand are called
A)Kawasaki fragments.
B)Nagasaki fragments.
C)Miyazaki fragments.
D)Okazaki fragments.
E)Suzuki fragments.

4As helicase unwinds the DNA molecule, the separated strands are kept apart by DNA polymerase.

5DNA replication can begin at any site along the DNA molecule.

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