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Steps in Cloning a Gene (Quiz 4)

Why are plasmids used in cloning genes? Plasmids are used in cloning genes because they are self-contained replicating entities. Each plasmid contains the instructions to copy the entire structure. Any gene inserted into a plasmid will be copied each time the plasmid is copied. A plasmid is small and physically separated from the rest of the genome. Isolating and extracting the gene of interest after it has been cloned is more practical if the gene is on a plasmid rather than on the bacterial chromosome.

View the animation below, then complete the quiz to test your knowledge of the concept.






1Unique restriction enzyme sites are found where in the plasmid?
A)the origin of replication
B)within the LacZ gene
C)within the selectable marker
D)all of the above



2Which of the following would produce blue colonies?
A)bacterial cells without plasmids
B)bacterial cells containing plasmids without inserts
C)bacterial cells containing plasmids with the gene of interest
D)bacterial cells containing plasmids with inserts, but not the gene of interest



3The presence of a selectable marker on the plasmid allows the researcher to determine if __________.
A)the bacterial cell has taken up the plasmid
B)the gene of interest has been inserted in the plasmid
C)the origin of replication is functioning
D)the beta-galactosidase gene is functioning



4DNA ligase will only recircularize plasmid DNA that contains the gene of interest to be cloned.
A)True
B)False



5Following insertion of a plasmid into competent cells, and plating of the cells on an ampicillin-betagalactosidase media, a researcher would select white colonies for additional study.
A)True
B)False







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