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FISH

View the animation below, then complete the quiz to test your knowledge of the concept.






1The term in situ is ________ for ________ .
A)Greek ; in cell
B)Greek ; in place
C)Greek ; in lab
D)Latin ; in cell
E)Latin ; in place



2The cells used in the FISH technique are first arrested during
A)interphase.
B)metaphase.
C)telophase.
D)prophase.
E)anaphase.



3Arrange the following in the proper sequence in which they occur during FISH.

1. Denaturation of DNA
2. Addition of DNA probes
3. Fixation of chromosomes
A)1, 2, 3
B)2, 1, 3
C)2, 3, 1
D)3, 2, 1
E)3, 1, 2



4In situ hybridization is used to detect the physical location of a gene on a chromosome.
A)True
B)False



5FISH probes are fluorescently-labeled and designed to bind only to non-complementary DNA.
A)True
B)False







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