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Restriction Endonucleases

View the animation below, then complete the quiz to test your knowledge of the concept.






1Which of the following could NOT be the recognition site of a restriction endonculease?
A)GAATTC
CTTAAG
B)ATCGAT
TAGCTA
C)CTGCAG
GACGTC
D)GCTTGC
CGAACG
E)GGATCC
CCTAGG



2The single-stranded ends of DNA molecules can be joined together by
A)restriction endonucleases.
B)DNA ligase.
C)DNA polymerase.
D)primase.
E)helicase.



3Human DNA cut with restriction enzyme A can be joined to
A)human DNA cut with restriction enzyme B.
B)human DNA that is uncut.
C)bacterial DNA cut with restriction enzyme A.
D)bacterial DNA that is uncut.
E)none of the above



4The joining of sticky ends involves the formation of phosphodiester bonds.
A)True
B)False



5Restriction enzymes cut only at specific sites and therefore are not useful for genetic engineering.
A)True
B)False







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