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Sanger Sequencing

View the animation below, then complete the quiz to test your knowledge of the concept.






1The primer used in Sanger sequencing
A)can have any nucleotide sequence
B)must have a sequence beginning and ending with the same nucleotide
C)has a nucleotide sequence complementary to the 3' end of the region to be copied
D)has a nucleotide sequence complementary to the 5' end of the region to be copied



2To carry out Sanger sequencing a mixture is needed containing
A)single-stranded DNA
B)DNA polymerase
C)four deoxyribonucleotides A, T, C, G
D)all of the above



3When a dideoxyribonucleotide is added to the tube
A)replication of the strand continues
B)replication of the strand stops
C)replication of the strand is not affected
D)replication of the strand is speeded up



4To sequence DNA by this method, the DNA must first be made in single stranded form.
A)True
B)False



5Examining the electrophoresis gel and reading from bottom to top, one base at a time, gives the sequence of DNA.
A)True
B)False







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