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ELISA Enzyme-Linked Immunosorbent Assay

View the animation below, then complete the quiz to test your knowledge of the concept.

1The direct ELISA test requires
A)known antibody
B)known antigen
D)patient antibody

2Place the following reactants in their proper order for the indirect ELISA test
1 = enzyme-linked antibody
2 = known antigen
3 = patient serum
4 = substrate
A)2 4 1 3
B)3 2 1 4
C)1 4 3 2
D)4 1 3 2
E)2 3 1 4

3In the indirect ELISA test the enzyme-linked antibody will attach to
A)the patient antigen
B)the variable region of the patient antibody
C)the constant region of the patient antibody
D)the wall of the microtiter well
E)known antibody

4In the indirect ELISA test the development of color means the patient has the antibody being tested for.

5The direct ELISA test is used to test for the presence of specific antibodies in a patient's serum.

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