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Construction of a Plasmid Vector

View the animation below, then complete the quiz to test your knowledge of the concept.






1In insertional inactivation
A)if foreign DNA is inserted, the beta galactosidase marker is inactivated, and the colonies remain white
B)if foreign DNA is inserted, the beta galactosidase marker is inactivated, and the colonies turn blue
C)if foreign DNA is inserted, the beta galactosidase marker is activated, and the colonies remain white
D)if foreign DNA is inserted, the beta galactosidase marker is activated, and the colonies turn blue



2If host cells are ampicillin sensitive and are plated on a medium containing ampicillin
A)only cells that have not taken up the ampicillin resistant vector can grow
B)only cells that have taken up the ampicillin resistant vector can grow
C)all cells will grow
D)ampicillin is inactivated



3The lac z gene marker codes for
A)b galactosidase, which splits x-gal
B)b galactosidase, which makes x-gal resistant to splitting
C)ampicillin resistance
D)white colonies



4When foreign DNA and plasmid are both cut with the same restriction enzyme and mixed together, all molecules will form recombinants.
A)True
B)False



5When beta galactosidase cleaves x-gal, white colonies result.
A)True
B)False







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