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Southern Blot

View the animation below, then complete the quiz to test your knowledge of the concept.

1The smaller the DNA fragment
A)the closer to the origin it will appear
B)the brighter color it produces with ethidium bromide
C)the faster it migrates during separation by electrophoresis
D)the slower it migrates during separation by electrophoresis

2The purpose of the Southern Blot test is to
A)look for a specific nucleotide sequence in the DNA being tested
B)to determine how closely two organisms are related
C)to identify the size of the fragment that contains the sequence
D)a and c
E)a, b, and c

3The steps involved in the Southern Blot test should be performed in the following order
1 = x-ray film
2 = electrophoresis
3 = digestion with restriction enzyme
4 = ethidium bromide
5 = radioactive probe
A)3, 2, 4, 5, 1
B)3, 4, 2, 5, 1
C)3, 2, 5, 4, 1
D)2, 4, 3, 5, 1

4The DNA probe will bind to identical nucleotide sequences on the test sample.

5The position of the bound radioactive probe is made visible on x-ray film.

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