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Immunofluorescence and RIA

View the animation below, then complete the quiz to test your knowledge of the concept.






1In immunofluorescence, the excess _____ is removed by _______.
A)antibody; precipitation
B)antibody; neutralization
C)antibody; washing
D)antigen; neutralization
E)antigen; washing



2Arrange the following steps in the proper sequence in which they occur during indirect immunofluorescence.
1. Addition of non-fluorescent antibody
2. Addition of fluorescent antibody
3. Fixation of antigen to slide
A)1, 2, 3
B)2, 1, 3
C)2, 3, 1
D)3, 2, 1
E)3, 1, 2



3The isotope used in the radioimmunoassay is
A)53I.
B)125I.
C)6C
D)14C.
E)35S.



4The immunofluorescence procedure requires the use of a pure culture.
A)True
B)False



5The radioimmunoassay procedure requires the use of a standard curve.
A)True
B)False







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